Janis Vogt, Ph.D. student in Gopal's lab, publishes a paper on the discovery and functional characterisation of FAM83G/PAWS1

Janis Vogt, Ph.D. student in Gopal's lab, publishes a paper on the discovery and functional characterisation of FAM83G/PAWS1
Janis Vogt, Ph.D. student in Gopal's lab, publishes a paper on the discovery and functional characterisation of FAM83G/PAWS1
Bone morphogenetic proteins (BMPs) control multiple cellular processes in embryos and adult tissues. BMPs signal through the activation of type I BMP receptor kinases, which then phosphorylate SMADs 1/5/8. In the canonical pathway, this triggers the association of these SMADs with SMAD4 and their translocation to the nucleus, where they regulate gene expression. BMPs can also signal independently of SMAD4, but this pathway is poorly understood.

Research undertaken primarily by Janis in Gopal Sapkota's laboratory over the last three years has uncovered unique roles of FAM83G/PAWS1 (Protein Associated With SMAD1) in the BMP signalling pathway and beyond.

The key novel findings that his research has made are highlighted below:

• PAWS1 selectively interacts with SMAD1

• PAWS1 forms a complex with SMAD1 in a SMAD4-independent manner

• PAWS1 is phosphorylated by BMPR1A in response to BMP, making it the first non-SMAD substrate to be reported.

• The phosphorylation of PAWS1 in response to BMP is essential for activation of the SMAD4-independent BMP target genes NEDD9 and ASNS.

• Additionally, PAWS1 regulates the expression of several non-BMP target genes, suggesting roles for PAWS1 beyond the BMP pathway.

Lina Herhaus, Robert Gourlay, Thomas Macartney, David Campbell as well as collaborators Kevin Dingwell and Jim Smith (NIMR, Mill Hill, London) also contributed to this research.

The paper entitled 'Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein

signalling' can be accessed through Open Biology.

[url">http://rsob.royalsocietypublishing.org/content/4/2/130210.short?rss=1

Janis successfully defended his Ph.D. thesis on 8th October 2013.

He is currently a postdoctoral research fellow in Prof. Thomas Jentsch's laboratory at the Max-Delbrueck-Center for Molecular Medicine/Leibniz-Institute for Molecular Pharmacology in Berlin, Germany. He is investigating the mechanisms of ion homeostasis of different anion and cations in cellular organelles in order to understand the underlying molecular mechanisms of ion channel associated diseases.

Reference:

Vogt J, Dingwell KS, Herhaus L, Gourlay R, Macartney T, Campbell D, Smith JC, Sapkota GP (2014) Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein signalling. Open Biol. 4:130210. [url">http://dx.doi.org/10.1098/rsob.130210