We have completely overhauled the MRC Protein Phosphorylation and Ubiquitylation Unit (MRC PPU) reagents and services website which lists all of the MRC-PPU's, cDNA clones, antibodies and recombinant proteins that have been generated over the years in the MRC PPU and enables researchers worldwide to request these for use in their own experiments.
We would invite you to have a look at the revamped site using the below link:
https://mrcppureagents.dundee.ac.uk
You just need to type in the name of the reagent that you are looking for and if we have it in our collection you will get the list of all of the different versions of cDNA clones sorted by vectors and epitope tags, antibodies and recombinant proteins we have for this reagent. These can then be easily requested through the website.
The website currently lists 24868 cDNA clones, 440 purified recombinant proteins and 412 antibodies. Our website is continuously updated with new reagents as an when they are generated.
The new website also lists collections of reagents available for different signalling pathways that our Units work on and that can easily be perused including:
LKB1 Pathway,
WNK-Pathway,
PI3K/PDK1/mTOR/AGC kinase Pathway,
TGFbeta and BMP Pathway
ERK Signalling Pathway
Components of the Ubiquitin Signalling Pathway (E1's, E2's, E3', DUBs and Ub's)
Interferon Signalling Pathway
DNA Replication Termination Signalling Pathway
Autophagy/Mitophagy Signalling Pathwa
Parkinson's Disease Pathways
Innate Immune Signalling Networks
There should be a comprehensive data-sheet for each reagent. If there isn't a data-sheet, for example for some constructs that were made many years ago we will re-sequence these constructs and generate a new data-sheet for these constructs as and when they are requested.
In addition, the website describes services that our Unit offers namely antibody generation (including antigen preparation and affinity purification of antibodies), recombinant protein expression and purification from both bacteria and insect cells as well as cDNA cloning services that includes generating constructs for CRISPR/CAS9 knock-out and knock-in projects
Finally, please feel free to forward suggestions to us on ideas for improvements that we can make to improve functionality or fix glitches.
If you have any suggestions for improvement please don't hesitate to e-mail your suggestions, however minor, to Matthew Elliott: m.elliott@dundee.ac.uk
We look forward to your continued support in 2016.
We would invite you to have a look at the revamped site using the below link:
https://mrcppureagents.dundee.ac.uk
You just need to type in the name of the reagent that you are looking for and if we have it in our collection you will get the list of all of the different versions of cDNA clones sorted by vectors and epitope tags, antibodies and recombinant proteins we have for this reagent. These can then be easily requested through the website.
The website currently lists 24868 cDNA clones, 440 purified recombinant proteins and 412 antibodies. Our website is continuously updated with new reagents as an when they are generated.
The new website also lists collections of reagents available for different signalling pathways that our Units work on and that can easily be perused including:
LKB1 Pathway,
WNK-Pathway,
PI3K/PDK1/mTOR/AGC kinase Pathway,
TGFbeta and BMP Pathway
ERK Signalling Pathway
Components of the Ubiquitin Signalling Pathway (E1's, E2's, E3', DUBs and Ub's)
Interferon Signalling Pathway
DNA Replication Termination Signalling Pathway
Autophagy/Mitophagy Signalling Pathwa
Parkinson's Disease Pathways
Innate Immune Signalling Networks
There should be a comprehensive data-sheet for each reagent. If there isn't a data-sheet, for example for some constructs that were made many years ago we will re-sequence these constructs and generate a new data-sheet for these constructs as and when they are requested.
In addition, the website describes services that our Unit offers namely antibody generation (including antigen preparation and affinity purification of antibodies), recombinant protein expression and purification from both bacteria and insect cells as well as cDNA cloning services that includes generating constructs for CRISPR/CAS9 knock-out and knock-in projects
Finally, please feel free to forward suggestions to us on ideas for improvements that we can make to improve functionality or fix glitches.
If you have any suggestions for improvement please don't hesitate to e-mail your suggestions, however minor, to Matthew Elliott: m.elliott@dundee.ac.uk
We look forward to your continued support in 2016.