We have overhauled the International Centre for Kinase Profiling website which lists all of the kinase profiling services that the MRC Protein Phosphorylation and Ubiquitylation Unit (MRC PPU) offers to enable researchers in both academic institutes and pharmaceutical companies to profile the specificity and potency of protein kinase inhibitors.
We would invite you to have a look at the revamped site using the below link:
[url">http://www.kinase-screen.mrc.ac.uk/
Kinase profiling services include
· MRC-PPU Premier Screen - Test your compounds against an extensive panel of 140 enzymes. This screen is undertaken once every 6 weeks and the data is provided 2 weeks from the screen start date.
· MRC-PPU Express Screen - Compounds can be screened against a subset of 50 kinases representing all areas of the human kinome. This screen is undertaken once every 3 weeks and the data will be provided 1 week from the screen start date.
· Lipid Kinase Screen - A panel of 16 lipid kinases undertaken once every 3 weeks with the data provided 1 week from the screen start date.
· IC50 Determination - If you have a compound you know inhibits a kinase or would like to follow up on a hit from a previous screen you can submit it to determine the IC50 value.
· ATP Competition Assay - Do you need to know if your compound of interest is a type I ATP competitive inhibitor?
· Substrate Screen - Do you have a purified kinase but no substrate to assay its activity?
· Custom Screening - Your compound(s) against your choice of kinase proteins available from our Premier Kinase Panel
The website also includes our widely used "Kinase Profiling Inhibitor Database'. This is a searchable database of specificities of nearly 250 commonly used signal transduction inhibitors screened against a panel of up to 140 kinases frequently at multiple concentrations. We strongly recommend undertaking kinase profiling of any kinase inhibitor to be used in a biological experiment. It is essential to have a good feel for the specificity of the kinase inhibitor you are working with in order to be able to properly interpret your data.To view and peruse data stored in the kinase profiling database click here.
The MRC PPU pioneered analysis of the selectivity of protein kinase inhibitors by setting up the first service to tackle this problem in 1998. This procedure, termed "kinase profiling" proved to be of great help to the pharmaceutical industry, speeding up the development of specific protein kinase inhibitors with therapeutic potential.
Protein kinases are one the pharmaceutical industry's most important class of drug target. Over 30 protein kinase inhibitors have been approved for clinical use and nearly 200 others undergoing clinical evaluation.
The International Centre for Kinase Profiling is operated by the MRC PPU and makes use of all of our huge in-house expertise in studying activity and function of protein kinases. We have been working with industrial partners since 1998 and this has translated into a level of rigor and attention to detail that makes The International Centre for Kinase Profiling a key asset to academia and industry-based scientists - at an affordable price.
We welcome your comments and suggestions on how to improve the site further. Please send all of your suggestions, no matter how minor, to kinase-screen@dundee.ac.uk
We would invite you to have a look at the revamped site using the below link:
[url">http://www.kinase-screen.mrc.ac.uk/
Kinase profiling services include
· MRC-PPU Premier Screen - Test your compounds against an extensive panel of 140 enzymes. This screen is undertaken once every 6 weeks and the data is provided 2 weeks from the screen start date.
· MRC-PPU Express Screen - Compounds can be screened against a subset of 50 kinases representing all areas of the human kinome. This screen is undertaken once every 3 weeks and the data will be provided 1 week from the screen start date.
· Lipid Kinase Screen - A panel of 16 lipid kinases undertaken once every 3 weeks with the data provided 1 week from the screen start date.
· IC50 Determination - If you have a compound you know inhibits a kinase or would like to follow up on a hit from a previous screen you can submit it to determine the IC50 value.
· ATP Competition Assay - Do you need to know if your compound of interest is a type I ATP competitive inhibitor?
· Substrate Screen - Do you have a purified kinase but no substrate to assay its activity?
· Custom Screening - Your compound(s) against your choice of kinase proteins available from our Premier Kinase Panel
The website also includes our widely used "Kinase Profiling Inhibitor Database'. This is a searchable database of specificities of nearly 250 commonly used signal transduction inhibitors screened against a panel of up to 140 kinases frequently at multiple concentrations. We strongly recommend undertaking kinase profiling of any kinase inhibitor to be used in a biological experiment. It is essential to have a good feel for the specificity of the kinase inhibitor you are working with in order to be able to properly interpret your data.To view and peruse data stored in the kinase profiling database click here.
The MRC PPU pioneered analysis of the selectivity of protein kinase inhibitors by setting up the first service to tackle this problem in 1998. This procedure, termed "kinase profiling" proved to be of great help to the pharmaceutical industry, speeding up the development of specific protein kinase inhibitors with therapeutic potential.
Protein kinases are one the pharmaceutical industry's most important class of drug target. Over 30 protein kinase inhibitors have been approved for clinical use and nearly 200 others undergoing clinical evaluation.
The International Centre for Kinase Profiling is operated by the MRC PPU and makes use of all of our huge in-house expertise in studying activity and function of protein kinases. We have been working with industrial partners since 1998 and this has translated into a level of rigor and attention to detail that makes The International Centre for Kinase Profiling a key asset to academia and industry-based scientists - at an affordable price.
We welcome your comments and suggestions on how to improve the site further. Please send all of your suggestions, no matter how minor, to kinase-screen@dundee.ac.uk