Structural and functional aspects of membrane trafficking linked to LRRK2 phosphorylation of Rab GTPases
Background
The most common familial and sporadic cases associated with Parkinson’s disease involve the Ser/Thr kinase LRRK2. The substrates of LRRK2 are a group of Rab GTPases that control diverse aspects of cellular dynamics including autophagy, exocytosis, ciliogenesis, and vesicle motility. Collaborative efforts from the labs of Amir Khan (Dublin, Ireland) and Dario Alessi (Dundee, Scotland) have identified several potentially exciting new protein complexes that may link LRRK2 activity to the molecular defects seen in Parkinson’s Disease. This project will involve the purification and structural characterization of protein complexes, as well as functional studies to understand how these complexes regulate membrane trafficking. Outstanding in-house resources at Dublin and Dundee include protein production facilities, biophysical instrumentation (ITC, SPR, light scattering), and access to mass spectrometry at the MRC PPU. In addition there is generous beamtime available at synchrotrons, as well as cryo-electron microscopy facilities.
Requirements
Candidates are expected to have experience in recombinant protein expression/purification and expertise in either X-ray structure determination or cryo-electron microscopy. The position is based in Dublin, Ireland but candidates are expected to be mobile and perform experiments in Dundee, Scotland, as needed during the term of the fellowship.
Fellowship
The gross salary for the position ranges from €43,907 - €51,677 depending on the level of experience. The position is available immediately and is funded for 3 years.
Further Information/Applications
Dr Amir Khan, School of Biochemistry and Immunology, Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland
email: amir.khan@tcd.ie
Application Procedure
Submit an electronic copy of Curriculum Vitae, including the names and addresses of at least two referees as well as a letter motivation:
Dr Amir Khan: amir.khan@tcd.ie
To apply on-line please visit: www.dundee.ac.uk/jobs. If you are unable to apply on-line please contact Human Resources on (01382) 386209 (answering machine) for an application pack. Please quote reference number
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