Lab Member | Gopal Sapkota

PhD Student

Gopal Sapkota Portrait
Past Lab Member
Date: October 1999 - December 2002

Current Position: Group Leader, MRC Protein Phosphorylation Unit, University of Dundee

Publications Generated While in the Lab:

1. Sapkota, G.P., Kieloch, A., Lizcano, J.M., Lain, S. Arthur, J.S.C., Williams, M.R., Morrice, N., Deak, M. and Alessi, D.R. (2001) Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90RSK and PKA but not its farnesylation at Cys433 are essential for LKB1 to suppress cell growth. J. Biol. Chem. 276, 19469-19482.

2.Jer-Lee, M., Thangada, S., Paik, J.H., Sapkota, G.P., Ancellin, N., Chae, S.S., Wu, M., Morales-Ruiz, M., Sessa, W.C. Alessi, D.R. & Hla, T. (2001) Akt mediated phosphorylation of the G-protein-coupled receptor EDG-1 is required for endothelial cell chemotaxis. Molecular Cell 8, 693-704.

3. Sapkota, G.P., Boudeau J., Deak, M., Kieloch, A., Morrice, N. & Alessi, D.R. (2002) Identification and characterisation of 4 novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome. Biochem. J 362, 481-490.

4. Douglas, P., Sapkota, G.P., Morrice, N., Yu, Y., Goodarzi, A.A., Merkle, D., Alessi, D.R. and Lees-Miller, S.P. (2002) Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase. Biochem. J. 368, 243-251.

5. Sapkota, G.P., Deak, M., Kieloch, A., Morrice, N., Goodarzi, A.A. Shiloh, Y., Lees-Miller S.P. and Alessi, D.R. (2002) Ionising radiation induces ATM-mediated phosphorylation of LKB1/STK11 at Thr366. Biochem. J. 368, 507-516.

6. Boudeau J., Sapkota G. P., Alessi D.R. (2003) LKB1, a protein kinase regulating cell proliferation and polarity. FEBS Lett.546:159-65.

7. Baas A.F., Boudeau J., Sapkota G.P., Smit L., Medema R., Morrice N.A., Alessi D.R., Clevers H.C. (2003) Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRAD. EMBO J. 22:3062-72.

8. Morgan, C.P., Skippen, A., Segui, B., Ball, A., Allen-Baume, V., Larijani, B., Murray-Rust, J., McDonald, N., Sapkota, G., Morrice, N. and Cockcroft, S. (2004) Phosphorylation of a distinct structural form of phosphatidylinositol transfer protein (alpha) at Ser166 by protein kinase C. J.Biol.Chem. 279, 47159-47171.

9. Sapkota, G.P., Cummingss, L., Newell, F.S., Armstrong, C., Bain, J., Frodin, F., Grauert, M., Hoffmann, M., Schnapp, G., Steegmaier, M., Cohen, P. and Alessi, D.R. (2006) BI-D1870 is a specific inhibitor of the p90 Ribosomal S6 Kinase (RSK) isoforms in vitro and in vivo. Biochemical Journal 401, 29-38.