Dates: July 2011 - June 2017

Current Position: Principle Investigator at the Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, Exeter, UK

Publications generated whilst in the lab:

1 Wang J, Erazo T, Ferguson FM, Buckley DL, Gomez N, Munoz-Guardiola P, Dieguez-Martinez N, Deng X, Hao M, Massefski W, Fedorov O, Offei-Addo NK, Park PM, Dai L, DiBona A, Becht K, Kim ND, McKeown MR, Roberts JM, Zhang J, Sim T, Alessi DR, Bradner JE, Lizcano JM, Blacklow SC, Qi J, Xu X, Gray NS. (2018) Structural and atropisomeric factors governing the selectivity profiles of pyrimido-benzodiazipinones as inhibitors of kinases and bromodomains. ACS Chem Biol. 13(9), 2438-2448

2 Heubl M, Zhang J, Pressey J, Renner M, Moutkine I, Russeau M, Eugene E, Kahle KT, Poncer JC, Levi S. (2017) GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the C1-sensitive WNK1 kinase. Nature Communications. 8(1), 1776

3 Shekarabi, M., Zhang, J., Khanna, A. R., Ellison, D. H., Delpire, E. and Kahle, K. T. (2017) WNK Kinase Signaling in Ion Homeostasis and Human Disease. Cell Metab. 25, 285-299

4 Kahle, K. T., Flores, B., Bharucha-Goebel, D., Zhang, J., Donkervoort, S., Hegde, M., Hussain, G., Duran, D., Liang, B., Sun, D., Bonnemann, C. G. and Delpire, E. (2016) Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter. Sci Signal. 9, ra77

5 Kahle, K. T., Schmouth, J. F., Lavastre, V., Latremoliere, A., Zhang, J., Andrews, N., Omura, T., Laganiere, J., Rochefort, D., Hince, P., Castonguay, G., Gaudet, R., Mapplebeck, J. C., Sotocinal, S. G., Duan, J., Ward, C., Khanna, A. R., Mogil, J. S., Dion, P. A., Woolf, C. J., Inquimbert, P. and Rouleau, G. A. (2016) Inhibition of the kinase WNK1/HSN2 ameliorates neuropathic pain by restoring GABA inhibition. Sci Signal. 9, ra32

6 Zhang, J., Deng, X. and Kahle, K. T. (2016) Leveraging unique structural characteristics of WNK kinases to achieve therapeutic inhibition. Sci Signal. 9, pe3

7 Zhang, J., Gao, G., Begum, G., Wang, J., Khanna, A. R., Shmukler, B. E., Daubner, G. M., de Los Heros, P., Davies, P., Varghese, J., Bhuiyan, M. I., Duan, J., Zhang, J., Duran, D., Alper, S. L., Sun, D., Elledge, S. J., Alessi, D. R. and Kahle, K. T. (2016) Functional kinomics establishes a critical node of volume-sensitive cation-Cl- cotransporter regulation in the mammalian brain. Sci. Rep. 6, 35986

8 Friedel, P., Kahle, K. T., Zhang, J., Hertz, N., Pisella, L. I., Buhler, E., Schaller, F., Duan, J., Khanna, A. R., Bishop, P. N., Shokat, K. M. and Medina, I. (2015) WNK1-regulated inhibitory phosphorylation of the KCC2 cotransporter maintains the depolarizing action of GABA in immature neurons. Sci Signal. 8, ra65

9 Hatcher, J. M., Zhang, J., Choi, H. G., Ito, G., Alessi, D. R. and Gray, N. S. (2015) Discovery of a Pyrrolopyrimidine (JH-II-127), a Highly Potent, Selective, and Brain Penetrant LRRK2 Inhibitor. ACS Med. Chem. Lett. 6, 584-589

10 Kazlauskaite, A., Martinez-Torres, R. J., Wilkie, S., Kumar, A., Peltier, J., Gonzalez, A., Johnson, C., Zhang, J., Hope, A. G., Peggie, M., Trost, M., van Aalten, D. M., Alessi, D. R., Prescott, A. R., Knebel, A., Walden, H. and Muqit, M. M. (2015) Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation. EMBO Rep. 16, 939-954

11 Schumacher FR, Siew K, Zhang J, Johnson C, Wood N, Cleary SE, Al Maskari RS, Ferryman JT, Hardege I, Yasmin, Figg NL, Enchev R, Knebel A, O'Shaughnessy KM, Kurz T.(2015) Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia. EMBO Mol Med. 7:1285-1306.

12 Zhang, J., Siew, K., Macartney, T., O'Shaughnessy, K. M. and Alessi, D. R. (2015) Critical role of the SPAK protein kinase CCT domain in controlling blood pressure. Hum. Mol. Genet. 24, 4545-4558

13 Alessi, D. R., Zhang, J., Khanna, A., Hochdorfer, T., Shang, Y. and Kahle, K. T. (2014) The WNK-SPAK/OSR1 pathway: Master regulator of cation-chloride cotransporters. Sci Signal. 7, re3

14 de Los Heros, P., Alessi, D. R., Gourlay, R., Campbell, D. G., Deak, M., Macartney, T. J., Kahle, K. T. and Zhang, J. (2014) The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters. Biochem. J. 458, 559-573

15 Delbroek, L., Van Kolen, K., Steegmans, L., da Cunha, R., Mandemakers, W., Daneels, G., De Bock, P. J., Zhang, J., Gevaert, K., De Strooper, B., Alessi, D. R., Verstreken, P. and Moechars, D. W. (2013) Development of an enzyme-linked immunosorbent assay for detection of cellular and in vivo LRRK2 S935 phosphorylation. J. Pharm. Biomed. Anal. 76C, 49-58

16 Deng, X., Elkins, J. M., Zhang, J., Yang, Q., Erazo, T., Gomez, N., Choi, H. G., Wang, J., Dzamko, N., Lee, J. D., Sim, T., Kim, N., Alessi, D. R., Lizcano, J. M., Knapp, S. and Gray, N. S. (2013) Structural determinants for ERK5 (MAPK7) and leucine rich repeat kinase 2 activities of benzo[e]pyrimido-[5,4-b]diazepine-6(11H)-ones. Eur. J. Med. Chem. 70, 758-767

17 Yao, C., Johnson, W. M., Gao, Y., Wang, W., Zhang, J., Deak, M., Alessi, D. R., Zhu, X., Mieyal, J. J., Roder, H., Wilson-Delfosse, A. L. and Chen, S. G. (2013) Kinase inhibitors arrest neurodegeneration in cell and C. elegans models of LRRK2 toxicity. Hum. Mol. Genet. 22, 328-344

18 Choi, H. G., Zhang, J., Deng, X., Hatcher, J. M., Patricelli, M. P., Zhao, Z., Alessi, D. R. and Gray, N. S. (2012) Brain Penetrant LRRK2 Inhibitor. ACS Med. Chem. Lett. 3, 658-662

19 Reith, A. D., Bamborough, P., Jandu, K., Andreotti, D., Mensah, L., Dossang, P., Choi, H. G., Deng, X., Zhang, J., Alessi, D. R. and Gray, N. S. (2012) GSK2578215A A potent and highly selective 2-arylmethyloxy-5-substitutent-N-arylbenzamide LRRK2 kinase inhibitor. Bioorg. Med. Chem. Lett. 22, 5625-5629

20 Zhang, J., Deng, X., Choi, H. G., Alessi, D. R. and Gray, N. S. (2012) Characterization of TAE684 as a potent LRRK2 kinase inhibitor. Bioorg. Med. Chem. Lett. 22, 1864-1869