Dates: 1997-2012

Current Position: Senior Molecular Biologist, Nestlé Institute of Health Sciences SA, Campus EPFL, Quartier de l'innovation, âtiment G, 1015 Lausanne, Switzerland

Publications generated while in lab:

SPAK/OSR1 regulate NKCC1 and WNK activity: analysis of WNK isoform interactions and activation by T-loop trans-autophosphorylation.

Thastrup JO, Rafiqi FH, Vitari AC, Pozo-Guisado E, Deak M, Mehellou Y, Alessi DR

Biochem J. 2012 Jan 1;441(1):325-37.

TTBK2 kinase substrate specificity and the impact of spinocerebellar-ataxia-causing mutations on expression, activity, localization and development.

Bouskila M, Esoof N, Gay L, Fang EH, Deak M, Begley MJ, Cantley LC, Prescott A, Storey KG, Alessi DR

Biochem J. 2011 Jul 1;437(1):157-67.

MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases.

Filippi BM, de los Heros P, Mehellou Y, Navratilova I, Gourlay R, Deak M, Plater L, Toth R, Zeqiraj E, Alessi DR

EMBO J. 2011 May 4;30(9):1730-41

Regulation of the NKCC2 ion cotransporter by SPAK-OSR1-dependent and -independent pathways.

Richardson C, Sakamoto K, de los Heros P, Deak M, Campbell DG, Prescott AR, Alessi DR

J Cell Sci. 2011 Mar 1;124(Pt 5):789-800

Temporal differences in the dependency on phosphoinositide-dependent kinase 1 distinguish the development of invariant Valpha14 NKT cells and conventional T cells.

Finlay DK, Kelly AP, Clarke R, Sinclair LV, Deak M, Alessi DR, Cantrell DA

J Immunol. 2010 Nov 15;185(10):5973-82

Inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser(910)/Ser(935), disruption of 14-3-3 binding and altered cytoplasmic localization.

Dzamko N, Deak M, Hentati F, Reith AD, Prescott AR, Alessi DR, Nichols RJ

Biochem J. 2010 Sep 15;430(3):405-13

14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization.

Nichols RJ, Dzamko N, Morrice NA, Campbell DG, Deak M, Ordureau A, Macartney T, Tong Y, Shen J, Prescott AR, Alessi DR

Biochem J. 2010 Sep 15;430(3):393-404

New roles for the LKB1-NUAK pathway in controlling myosin phosphatase complexes and cell adhesion.

Zagórska A, Deak M, Campbell DG, Banerjee S, Hirano M, Aizawa S, Prescott AR, Alessi DR

Sci Signal. 2010 Mar 30;3(115):ra25

Structure of the LKB1-STRAD-MO25 complex reveals an allosteric mechanism of kinase activation.

Zeqiraj E, Filippi BM, Deak M, Alessi DR, van Aalten DM

Science. 2009 Dec 18;326(5960):1707-11

Substrate specificity and inhibitors of LRRK2, a protein kinase mutated in Parkinson's disease.

Nichols RJ, Dzamko N, Hutti JE, Cantley LC, Deak M, Moran J, Bamborough P, Reith AD, Alessi DR

Biochem J. 2009 Oct 23;424(1):47-60

ATP and MO25alpha regulate the conformational state of the STRADalpha pseudokinase and activation of the LKB1 tumour suppressor.

Zeqiraj E, Filippi BM, Goldie S, Navratilova I, Boudeau J, Deak M, Alessi DR, van Aalten DM

PLoS Biol. 2009 Jun 9;7(6):e1000126

Structure of the OSR1 kinase, a hypertension drug target.

Villa F, Deak M, Alessi DR, van Aalten DM

Proteins. 2008 Dec;73(4):1082-7

Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains.

Al-Hakim AK, Zagorska A, Chapman L, Deak M, Peggie M, Alessi DR

Biochem J. 2008 Apr 15;411(2):249-60

Structural insights into the recognition of substrates and activators by the OSR1 kinase.

Villa F, Goebel J, Rafiqi FH, Deak M, Thastrup J, Alessi DR, van Aalten DM

EMBO Rep. 2007 Sep;8(9):839-45

Identification of Protor as a novel Rictor-binding component of mTOR complex-2.

Pearce LR, Huang X, Boudeau J, Pawłowski R, Wullschleger S, Deak M, Ibrahim AF, Gourlay R, Magnuson MA, Alessi DR

Biochem J. 2007 Aug 1;405(3):513-22

LRRK2 phosphorylates moesin at threonine-558: characterization of how Parkinson's disease mutants affect kinase activity.

Jaleel M, Nichols RJ, Deak M, Campbell DG, Gillardon F, Knebel A, Alessi DR

Biochem J. 2007 Jul 15;405(2):307-17

Regulation of activity and localization of the WNK1 protein kinase by hyperosmotic stress.

Zagórska A, Pozo-Guisado E, Boudeau J, Vitari AC, Rafiqi FH, Thastrup J, Deak M, Campbell DG, Morrice NA, Prescott AR, Alessi DR

J Cell Biol. 2007 Jan 1;176(1):89-100

Identification of novel phosphorylation sites in MSK1 by precursor ion scanning MS.

McCoy CE, macdonald A, Morrice NA, Campbell DG, Deak M, Toth R, McIlrath J, Arthur JS.

Biochem J. 2007 Mar 15;402(3):491-501

Regulation of the polarity kinases PAR-1/MARK by 14-3-3 interaction and phosphorylation.

Göransson O, Deak M, Wullschleger S, Morrice NA, Prescott AR, Alessi DR

J Cell Sci. 2006 Oct 1;119(Pt 19):4059-70

Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1.

Vitari AC, Thastrup J, Rafiqi FH, Deak M, Morrice NA, Karlsson HK, Alessi DR

Biochem J. 2006 Jul 1;397(1):223-31

The ubiquitin-associated domain of AMPK-related kinases regulates conformation and LKB1-mediated phosphorylation and activation.

Jaleel M, Villa F, Deak M, Toth R, Prescott AR, Van Aalten DM, Alessi DR

Biochem J. 2006 Mar 15;394(Pt 3):545-55

14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK.

Al-Hakim AK, Göransson O, Deak M, Toth R, Campbell DG, Morrice NA, Prescott AR, Alessi DR

J Cell Sci. 2005 Dec 1;118(Pt 23):5661-73

In vivo role of the phosphate groove of PDK1 defined by knockin mutation.

Collins BJ, Deak M, Murray-Tait V, Storey KG, Alessi DR

J Cell Sci. 2005 Nov 1;118(Pt 21):5023-34

The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases.

Vitari AC, Deak M, Morrice NA, Alessi DR

Biochem J. 2005 Oct 1;391(Pt 1):17-24

Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding.

Komander D, Kular G, Deak M, Alessi DR, van Aalten DM

J Biol Chem. 2005 May 13;280(19):18797-802

Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate.

Jaleel M, McBride A, Lizcano JM, Deak M, Toth R, Morrice NA, Alessi DR

FEBS Lett. 2005 Feb 28;579(6):1417-23

Crystal structure of the PTPL1/FAP-1 human tyrosine phosphatase mutated in colorectal cancer: evidence for a second phosphotyrosine substrate recognition pocket.

Villa F, Deak M, Bloomberg GB, Alessi DR, van Aalten DM

J Biol Chem. 2005 Mar 4;280(9):8180-7

MSK1 activity is controlled by multiple phosphorylation sites.

McCoy CE, Campbell DG, Deak M, Bloomberg GB, Arthur JS

Biochem J. 2005 Apr 15;387(Pt 2):507-17

Analysis of the LKB1-STRAD-MO25 complex.

Boudeau J, Scott JW, Resta N, Deak M, Kieloch A, Komander D, Hardie DG, Prescott AR, van Aalten DM, Alessi DR

J Cell Sci. 2004 Dec 15;117(Pt 26):6365-75

Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates.

Komander D, Fairservice A, Deak M, Kular GS, Prescott AR, Peter Downes C, Safrany ST, Alessi DR, van Aalten DM

EMBO J. 2004 Oct 13;23(20):3918-28

LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1.

Lizcano JM, Göransson O, Toth R, Deak M, Morrice NA, Boudeau J, Hawley SA, Udd L, Mäkelä TP, Hardie DG, Alessi DR

EMBO J. 2004 Feb 25;23(4):833-43

Interactions of LY333531 and other bisindolyl maleimide inhibitors with PDK1.

Komander D, Kular GS, Schüttelkopf AW, Deak M, Prakash KR, Bain J, Elliott M, Garrido-Franco M, Kozikowski AP, Alessi DR, van Aalten DM

Structure. 2004 Feb;12(2):215-26

Purification, crystallization and preliminary X-ray diffraction of a proteolytic fragment of PDK1 containing the pleckstrin homology domain.

Komander D, Deak M, Morrice N, van Aalten DM

Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):314-6

Crystal structure of MO25 alpha in complex with the C terminus of the pseudo kinase STE20-related adaptor.

Milburn CC, Boudeau J, Deak M, Alessi DR, van Aalten DM

Nat Struct Mol Biol. 2004 Feb;11(2):193-200

WNK1, the kinase mutated in an inherited high-blood-pressure syndrome, is a novel PKB (protein kinase B)/Akt substrate.

Vitari AC, Deak M, Collins BJ, Morrice N, Prescott AR, Phelan A, Humphreys S, Alessi DR

Biochem J. 2004 Feb 15;378(Pt 1):257-68

MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localize LKB1 in the cytoplasm.

Boudeau J, Baas AF, Deak M, Morrice NA, Kieloch A, Schutkowski M, Prescott AR, Clevers HC, Alessi DR

EMBO J. 2003 Oct 1;22(19):5102-14

Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1.

Kimber WA, Deak M, Prescott AR, Alessi DR

Biochem J. 2003 Dec 1;376(Pt 2):525-35

Binding of phosphatidylinositol 3,4,5-trisphosphate to the pleckstrin homology domain of protein kinase B induces a conformational change.

Milburn CC, Deak M, Kelly SM, Price NC, Alessi DR, Van Aalten DM

Biochem J. 2003 Nov 1;375(Pt 3):531-8

In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.

Collins BJ, Deak M, Arthur JS, Armit LJ, Alessi DR

EMBO J. 2003 Aug 15;22(16):4202-11

Insulin-induced Drosophila S6 kinase activation requires phosphoinositide 3-kinase and protein kinase B.

Lizcano JM, Alrubaie S, Kieloch A, Deak M, Leevers SJ, Alessi DR.

Biochem J. 2003 Sep 1;374(Pt 2):297-306

Heat-shock protein 90 and Cdc37 interact with LKB1 and regulate its stability.

Boudeau J, Deak M, Lawlor MA, Morrice NA, Alessi DR

Biochem J. 2003 Mar 15;370(Pt 3):849-57

Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366.

Sapkota GP, Deak M, Kieloch A, Morrice N, Goodarzi AA, Smythe C, Shiloh Y, Lees-Miller SP, Alessi DR

Biochem J. 2002 Dec 1;368(Pt 2):507-16

High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site.

Biondi RM, Komander D, Thomas CC, Lizcano JM, Deak M, Alessi DR, van Aalten DM

EMBO J. 2002 Aug 15;21(16):4219-28

Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo.

Lizcano JM, Deak M, Morrice N, Kieloch A, Hastie CJ, Dong L, Schutkowski M, Reimer U, Alessi DR

J Biol Chem. 2002 Aug 2;277(31):27839-49

Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome.

Sapkota GP, Boudeau J, Deak M, Kieloch A, Morrice N, Alessi DR

Biochem J. 2002 Mar 1;362(Pt 2):481-90

Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo.

Kimber WA, Trinkle-Mulcahy L, Cheung PC, Deak M, Marsden LJ, Kieloch A, Watt S, Javier RT, Gray A, Downes CP, Lucocq JM, Alessi DR

Biochem J. 2002 Feb 1;361(Pt 3):525-36

Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity.

Thomas CC, Dowler S, Deak M, Alessi DR, van Aalten DM

Biochem J. 2001 Sep 1;358(Pt 2):287-94

The PIF-binding pocket in PDK1 is essential for activation of S6K and SGK, but not PKB.

Biondi RM, Kieloch A, Currie RA, Deak M, Alessi DR

EMBO J. 2001 Aug 15;20(16):4380-90

Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90(RSK) and cAMP-dependent protein kinase, but not its farnesylation at Cys(433), is essential for LKB1 to suppress cell vrowth.

Sapkota GP, Kieloch A, Lizcano JM, Lain S, Arthur JS, Williams MR, Morrice N, Deak M, Alessi DR

J Biol Chem. 2001 Jun 1;276(22):19469-82

Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities.

Dowler S, Currie RA, Campbell DG, Deak M, Kular G, Downes CP, Alessi DR

Biochem J. 2000 Oct 1;351(Pt 1):19-31

A 3-phosphoinositide-dependent protein kinase-1 (PDK1) docking site is required for the phosphorylation of protein kinase Czeta (PKCzeta ) and PKC-related kinase 2 by PDK1.

Balendran A, Biondi RM, Cheung PC, Casamayor A, Deak M, Alessi DR

J Biol Chem. 2000 Jul 7;275(27):20806-13

Identification of a pocket in the PDK1 kinase domain that interacts with PIF and the C-terminal residues of PKA.

Biondi RM, Cheung PC, Casamayor A, Deak M, Currie RA, Alessi DR

EMBO J. 2000 Mar 1;19(5):979-88

Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase.

Kobayashi T, Deak M, Morrice N, Cohen P

Biochem J. 1999 Nov 15;344 Pt 1:189-97

Characterisation of a plant 3-phosphoinositide-dependent protein kinase-1 homologue which contains a pleckstrin homology domain.

Deak M, Casamayor A, Currie RA, Downes CP, Alessi DR

FEBS Lett. 1999 May 28;451(3):220-6

PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2.

Balendran A, Casamayor A, Deak M, Paterson A, Gaffney P, Currie R, Downes CP, Alessi DR

Curr Biol. 1999 Apr 22;9(8):393-404

Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1.

Currie RA, Walker KS, Gray A, Deak M, Casamayor A, Downes CP, Cohen P, Alessi DR, Lucocq J

Biochem J. 1999 Feb 1;337 ( Pt 3):575-83

Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB.

Deak M, Clifton AD, Lucocq LM, Alessi DR

EMBO J. 1998 Aug 3;17(15):4426-41

Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha.

Walker KS, Deak M, Paterson A, Hudson K, Cohen P, Alessi DR

Biochem J. 1998 Apr 1;331 ( Pt 1):299-308

3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase.

Alessi DR, Deak M, Casamayor A, Caudwell FB, Morrice N, Norman DG, Gaffney P, Reese CB, MacDougall CN, Harbison D, Ashworth A, Bownes M

Curr Biol. 1997 Oct 1;7(10):776-89